Plasmid_Backbone
Part:BBa_K260002:Design
Designed by: Kaj Bernhardt Group: iGEM09_BIOTEC_Dresden (2009-10-17)
pTetFlp. [anhydrotetracycline] -> FLP(F70L)
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 3222
Illegal EcoRI site found at 5762
Illegal XbaI site found at 67
Illegal XbaI site found at 6532
Illegal SpeI site found at 748
Illegal SpeI site found at 5444
Illegal PstI site found at 3132
Illegal PstI site found at 4756
Illegal PstI site found at 6283 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3222
Illegal EcoRI site found at 5762
Illegal EcoRI site found at 6680
Illegal NheI site found at 1771
Illegal SpeI site found at 2
Illegal SpeI site found at 748
Illegal SpeI site found at 5444
Illegal PstI site found at 16
Illegal PstI site found at 3132
Illegal PstI site found at 4756
Illegal PstI site found at 6283
Illegal NotI site found at 9
Illegal NotI site found at 6686 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3222
Illegal EcoRI site found at 5762
Illegal EcoRI site found at 6680
Illegal BglII site found at 4969
Illegal XhoI site found at 61
Illegal XhoI site found at 6674 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found at 6680
Illegal suffix found at 2
Illegal EcoRI site found at 3222
Illegal EcoRI site found at 5762
Illegal XbaI site found at 67
Illegal XbaI site found at 6532
Illegal SpeI site found at 748
Illegal SpeI site found at 5444
Illegal PstI site found at 3132
Illegal PstI site found at 4756
Illegal PstI site found at 6283 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 6680
Plasmid lacks a suffix.
Illegal EcoRI site found at 3222
Illegal EcoRI site found at 5762
Illegal XbaI site found at 67
Illegal XbaI site found at 6532
Illegal XbaI site found at 6695
Illegal SpeI site found at 2
Illegal SpeI site found at 748
Illegal SpeI site found at 5444
Illegal PstI site found at 16
Illegal PstI site found at 3132
Illegal PstI site found at 4756
Illegal PstI site found at 6283
Illegal NgoMIV site found at 3927
Illegal NgoMIV site found at 4305 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal SapI site found at 4245
Illegal SapI site found at 4455
Design Notes
dhfr had to be deleted as the same gene for trimpethoprim resistance was going to be used elsewhere in the same cell.
Source
This plasmid comes from Sarov M, Schneider S, Pozniakovski A, Roguev A, Ernst S, Zhang Y, Hyman AA, Stewart AF. A recombineering pipeline for functional genomics applied to Caenorhabditis elegans. Nat Methods. 2006 Oct;3(10):839-44. However, it was modified by deletion of dhfr gene and RedABGRecA operon through insertion of a kanamycin resistance cassette.